Research Article | | Peer-Reviewed

Reproductive Biotechnology Tools as a Game Changer for the Dairy Sector: The Case of Sexed Semen and Estrus Synchronization to Produce Seedstock Dairy Heifers

Received: 27 April 2024     Accepted: 20 May 2024     Published: 29 July 2024
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Abstract

Human curiosity about the sexual orientation of living things has a long history. For thousands of years, livestock owners wanted to find a suitable way to predict the sex of the progeny to be born for their herds on their farm. Thanks to the development of semen sexing technology, animal breeding has undergone a revolution, which allows farmers to control the sex of their offspring. Sexed semen enhances overall productivity and also assists farmers in meeting the rising demand for high-quality dairy animals by enabling them to selectively generate seedstock female calves. The technology is a one-stop solution to enhance breed improvement especially in a country like Ethiopia where 97 percent of the cattle population is indigenous, which is not selected for milk production and results in undernourishment for animal source origin diet, including milk and meat. The benefits of using sexed semen include sex selection, improved genetic selection, enhanced breeding effectiveness, better control over herd dynamics, greater financial gains, environmental sustainability, and improved animal welfare. Hence, with the finding of this study, an overall result of a 98% response rate 95% female skewness, and 79% conception rate was recorded after being inseminated with sexed semen. The finding of the response rate is closer to the results reported by another researcher, while the average conception rate is comparatively higher. This could be due to careful animal selection, the use of fertile quality semen, proper heat detection, and inseminating at an optimum window of time. In general, from the piece of this study, it is possible to foresee and conclude that the adoption and application of sexed semen technology is a groundbreaking and game-changer technology for the dairy industry in a country like Ethiopia, which overcomes the limitations of improved crossbred dairy cattle, replacement heifers, and high milk prices on the one hand, a shortage of grazing land, and environmental destruction due to a decreasing number of unwanted cattle population on the other side.

Published in International Journal of Animal Science and Technology (Volume 8, Issue 3)
DOI 10.11648/j.ijast.20240803.11
Page(s) 30-37
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Dairy Industry, Game Changer, Seedstock Dairy Heifers, Sexed Semen

1. Introduction
Human curiosity about the sexual orientation of living things has a long history. For thousands of years, livestock owners wanted to find a suitable way to predict the sex of the progeny to be born for their herds on their farm. This dates back to the early Greeks, specifically Democritus (470–402 BC), who believed that the right testis produces males while the left testis produces females.
However, the advancement in the biological sciences, particularly genetics, led to many discoveries during the first half of the 20th century, one of which was the identification of the sex chromosomes. Accordingly, improvement in livestock productivity could be achieved through selective breeding and control of production . Artificial insemination (AI), the oldest and most effective reproductive technology with estrous synchronization, is a regular practice on large-scale animal farms, and desired-sex animals are in high demand in such animal forms . Dairy animal keepers demand female cattle, whereas male calves are required for meat products for the beef sector. The ideal method for sex control in farm animals is the separation of “X” and “Y” chromosome-bearing live sperm cells from the natural mixture of semen samples.
Even though numerous traditional methods of sperm sorting have been developed, A recently developed flow cytometry technique increases the potential of commercially available sex-sorted semen. Normally, while using conventional semen, on average over thousands of animals, 49% of calves born will be heifers, and a few will be sterile freemartins Currently, the supply of replacement heifers on dairy farms is a major issue in commercial ventures.
Sex-sorted bovine semen became commercially available in the early 2000s. The application of sexed semen technology has gained importance in the bovine (beef and dairy) industry due to the high demand for milk and meat by the growing population of the world . This technology is used to reduce animal-derived food scarcity on the one hand and to improve the economy of animal farmers on the other . On the other hand, sexed semen technology is important in reducing the number of animals with unwanted sex in the beef or dairy sector, and this will greatly reduce the maintenance cost of the animal farm .
The flow cytometric approach of the sperm cell sorting technique is sorting the X chromosome-bearing sperm and Y chromosome-bearing sperm based on differences in their DNA content . The differences in DNA content in domestic animals between X and Y chromosome-bearing spermatozoa range from 3% to 4.5% , and while using a fluorescent dye that binds to DNA, male and female cattle sperm that carry different chromosomes can be electrically charged. This makes it possible for a fluorescence-activated cell sorter to separate them . About 90% of the sperm contain the desired sex, indicating that the procedure is reasonably accurate .
In Ethiopia, where 97% of the total cattle population is indigenous and is not selected for milk production, the introduction of assisted reproductive technologies like sexed semen and estrus synchronization technologies will be considered a game changer for the sector. Therefore, keeping all facts into consideration, the objectives of this research work are to assess the efficacy of the CIDR-based estrus synchronization technique and ascertain the potential of sexed semen technology in producing seedstock heifers for the dairy sector.
2. Material and Methods
2.1. Description of Study Area
This study was carried out from August 2020 to November 2023. The experiment was conducted both at a research station, the Debre Zeit Agricultural Research Centre (DZARC), with the National Animal Biotechnology Research Program of the Ethiopian Institute of Agricultural Research (EIAR), and at a private dairy farm found in Bishoftu City, Dukem, Bishoftu and Oda Bultum University located in Oromia regional state, is located about 45 km east of Addis Ababa, the capital city of Ethiopia (8°46′13.57"N, 38°59′50.45"E) at an altitude of 1920 m.a.s.l.
2.2. Experimental Animals
Indigenous Boran breed and high-grade Boran crossed Holstein Friesian heifers and cows were used for this study. All selected animals are cycling and had a body condition score (BCS) of 3- 8 on a scale of 1 to 9; (when 1=emaciated; 9 =obese). All the experimental animals were maintained as a group and were housed in a semi-opened housing system. Experimental animals were provided with a feed of different mixes: tef (Eragrostis tef) straw and grass (Andropogon abyssinicus) hay as a basal diet and supplemented with commercially prepared concentrate, mineral salts, and alfalfa green fodder. Management of these animals was nearly similar and sometimes DZARC experimental animals were released extensively for free grazing. Water was provided ad libitum. The experimental animals were regularly dewormed against a common parasitic disease and vaccinated for lumpy skin disease (LSD), foot and mouth disease (FMD), and other common infections.
2.3. Experimental Protocol
In the first trial, from the research center, a total of 104 animals were gynecologically examined with transrectal palpation for the presence of a well-developed and functional corpus luteum (CL) in either ovary, from the research center, university, and private farms. A total of 80 animals were technically selected and Control Intravaginal Drug Release (CIDR) devices were implanted for seven days for synchronization. A Zoetis product, luteolytic hormone (Lutalyse® injection dinoprost tromethamine), was injected deep intramuscularly on day six, post-CIDR implantation. Crayon livestock paint and ESTROTECT, a red or orange fluorescent color-coated heat detector, were applied on day seven during CIDR removal on the halfway point between the hip and tail heads perpendicular to the spine after brushing the hair thoroughly to create an optimal condition for adhesion. Hence, with each mount, the surface will gradually turn from silver to its indicator color, indicating true standing heat. As per the manufacturer's recommendation, approximately 50% of the silver rub-off coat removed should indicate standing heat. Sexed semen from ABS Global was used for insemination. Since sexed semen remains fertile for a shorter period in the female reproductive tract following insemination, insemination was conducted approximately 18 to 24 hours after the start of that female’s standing heat behavior (Figure 1).
In the second trial, a total of 40 heifers born from the first batch at the research center and private farm were gynecologically examined with transrectal palpation for the presence of a well-developed and functional CL in either ovary, and 35 heifers were technically selected. CIDR devices were implanted for seven days for synchronization (table 2).
Figure 1. Synchronization and artificial insemination protocol.
Figure 2. a & b) CIDR and its applicator and c) ESTROTECT d) Crayon livestock paint for heat detection.
Figure 3. First batch synchronized animals manifesting behavioral estrus sign.
3. Results
During the first shoot of this study, in the research center, governmental university, and on a private farm, a total, of 104 animals were gynecologically examined, and screened animals were selected and synchronized. From the synchronized animals, those responded animals were inseminated using sex-sorted semen. The breed, conception rate, sex of newborn calves, or gender skewness at all farms were recorded. The overall response rate, conception rate, and sex skewness. was 98%, 79%, and 95% respectively.
Table 1. Estrus response, conception rate, and calve sex ratio in cattle inseminated with sexed semen at the research center's private dairy farm and universities.

Farm category

Gynecologically examined animals

Synchronized (CIDR) implanted animals

Animal responded

Inseminated animals

Pregnant animal

Abortion and calve mortality encountered

Female skewness (%)

Research center

HF

31

25

24

24

20

2

100% (18/18)

B

7

5

5

5

3

0

67% (2/3)

Private farm

HF

31

26

26

25

22

0

91% (20/22)

University

HF

35

24

23

23

18

0

100% (18/18)

Total

104

80

78 (98%)

77

61 (79)

2

95% (58/61)

HF: Holstein Friesian breed; B=Boran Breed
Figure 4. Calves born on research station and private dairy farm using sexed semen (F1).
During the second phase of the experiment, heifers born from the first batch were synchronized once they reached puberty and inseminated using sexed semen following the same procedure as in the first phase experiment and given female calve crop (Figure 5 and Figure 6).
Table 2. Estrus response, conception rate, and calve sex ratio in heifers born from sexed semen and inseminated with sexed semen at the research center, private dairy farm, and universities.
Figure 5. Heifers born from sexed semen give female calve crops (seedstock) after estrus synchronized and inseminated with sexed semen (F2).
Figure 6. Collective figure for female calves born from whose mothers were born with sexed semen (F2) at DZARC.
4. Discussion
In a country like Ethiopia, where about 97% of the total cattle population is indigenous and is not selected for milk production due to low milk yielders which is below 1.5 liters per day, producing crossbred heifers through sexed semen technology is not a choice rather mandatory.
In several large-scale studies with the use of sexed semen in cows and heifers as well as within heifers in dairy and beef heifers, the conception rates are different. When using sexed semen compared to conventional semen in beef heifers, a 4% to 38% reduction in pregnancy rates were reported . Similarly, reported that the pregnancy rate of heifers inseminated with sexed semen resulted in a 17% decrease in pregnancy rates compared to heifers inseminated with conventional semen. This is mainly due to two main reasons, these are, 1). Due to the effects of the sorting procedure, and 2). low number of sperm in a standard dose of sorted semen is responsible for the reduction in fertility. According to a low number of spermatozoa accounts for two-thirds of the reduction in the pregnancy rate whereas damage caused by the sorting accounts for one-third.
Regarding the finding of this study, In the first trial, of 104 gynecologically examined and screened animals from research centers, private farmers, and universities, 80 animals were selected and synchronized with the CIDR plus prostaglandin-based synchronization protocol. An overall result of a 98% response rate 95% female skewness and 79% conception rate was recorded after being inseminated with sexed semen. The finding of the response rate is closer to the results reported by which is about a 100% response rate but in contrast to other reports like where 30 and 76.47% estrus expressions were reported respectively. With regards to female skewness, the current result is higher compared to , findings that are about 82.14% of female calves, and where 85–90% of desired sex calves were born by sexed semen.
The average conception rate of sexed semen was found to be 79%, which is comparatively higher than many previous reports, which stated that the fertility of sexed semen reached about 75–85% of the unsexed semen and some trials of . This could be due to the cows being fertile, which are selected purposefully, the semen being fertile, which is obtained from ABS Global, proper heat detection using herd attendants and ESTROTECT heat detectors, and researcher-based inseminations being conducted at the optimum window of time.
For the second trial, about 40 heifers born from the first batch of sexed semen insemination at the research center, private farm, and university (Figure 4) were gynecologically examined and 35 were synchronized and inseminated with sexed semen, resulting in a 94% response rate (table 2), which is similar to the result of the first trial.
The main application of sex-sorted semen is producing a potential dairy heifer (seedstock) for future quality and highly productive herd replacement. Beyond this, there are several advantages to using sexed semen in the dairying system. 1st the fertility of heifers is superior to that of lactating cows. 2nd the ease of calving smaller and lighter female calves and thereby the occurrence of less dystocia compared with giving birth to male calves . steadily producing the replacements from heifers, shortens the generation interval. 4th, herd expansion and the sale of heifers to others. 5th Economic aspect (raising replacement heifers at a lower cost than purchasing them). 6th building known genetics and 7th biosecurity issues (including managing Johne’s disease).
Since the best genes in a herd are among the youngest animals, the genetic progress is accelerated when they are used to produce the next generation. Furthermore, one interesting advantage of using sexed semen and also realized in this particular study is a heifer born from sexed semen gives a heifer through sexed semen insemination which has created a stir in the dairy industry.
5. Conclusion and Recommendation
The dairy industry is ever-changing as technology alters management practices. At present, purebred and commercial seedstock producers will receive the most benefit from the use of sexed semen. In addition, the use of sexed semen by commercial producers to generate replacement heifers or to breed replacement heifers is a viable option. In this article, scenarios for using sexed semen have been critically evaluated.
In general, from the piece of this study, it is possible to foresee the adoption and application of sexed semen technology as a groundbreaking and game-changer technology for the dairy industry in a country like Ethiopia where there is an insignificant number of improved crossbred dairy cattle, which results in skyrocketing prices for replacement heifers, high milk prices, and the shortage of heifers for replacements is the only solution. Furthermore, rapid population growth, and urbanization, result in a shrinkage of grazing land, and environmental destruction due to the greater number versus low productive cattle population.
In conclusion, sex-sorted semen has revolutionized animal breeding by providing farmers with the desired sex of calve offspring, better control over herd dynamics, and greater financial gains. Overall, semen sexing offers tremendous potential for genetic advancement, improved herd management, and meeting the demands of the improved livestock potential specifically and agricultural industry at large.
Abbreviations

AI

Artificial Insemination

BCS

Body Condition Score

CIDR

Control Intravaginal Drug Release

CL

Corpus Luteum

DNA

Deoxyribonucleic Acid

DZARC

Debre Zeit Agricultural Research Center

LSD

Lumpy Skin Disease

FM

Foot and Mouth Disease

ABS

American Breeder Services

Acknowledgments
The authors sincerely thank the Ethiopian Institute of Agriculture (EIAR), and Debre Zeit Agricultural Research for providing research fund and all the needed logistics. Furthermore, the authors express their gratitude to Professor Curt. R. Youngs for donating a synchronization hormone, CIDR, and sexed semen.
Author Contributions
Sayid Ali: Conceptualizing research ideas, acquiring funding, developing methodologies, managing projects, procuring resources, and participating practically in field activities of the project, supervising, writing the first draft, and editing
Tamrat Degefa: Conceptualizing research ideas, participating practically in field activities, organizing data, conducting formal analysis, using software, and assessing and revising draft manuscripts
Asnaku Funga: Practical engaging on field activity, methodology designing, validation, Data collection and curation, Writing, review & editing
Mosisa Dire: Practical engaging on field activity, Data collection and curation, Methodology designing, Validation, Visualization, Writing, review & editing
Ayda Mohamed: Practical engaging on field activity, Methodology Validation, Data collection and curation
Asmarech Yeshaneh: Practical engaging on field activity, Methodology Validation, Data collection and curation
Conflicts of Interest
The authors declare no conflicts of interest.
References
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    Ali, S., Degefa, T., Funga, A., Dire, M., Mohamed, A., et al. (2024). Reproductive Biotechnology Tools as a Game Changer for the Dairy Sector: The Case of Sexed Semen and Estrus Synchronization to Produce Seedstock Dairy Heifers. International Journal of Animal Science and Technology, 8(3), 30-37. https://doi.org/10.11648/j.ijast.20240803.11

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    Ali, S.; Degefa, T.; Funga, A.; Dire, M.; Mohamed, A., et al. Reproductive Biotechnology Tools as a Game Changer for the Dairy Sector: The Case of Sexed Semen and Estrus Synchronization to Produce Seedstock Dairy Heifers. Int. J. Anim. Sci. Technol. 2024, 8(3), 30-37. doi: 10.11648/j.ijast.20240803.11

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    Ali S, Degefa T, Funga A, Dire M, Mohamed A, et al. Reproductive Biotechnology Tools as a Game Changer for the Dairy Sector: The Case of Sexed Semen and Estrus Synchronization to Produce Seedstock Dairy Heifers. Int J Anim Sci Technol. 2024;8(3):30-37. doi: 10.11648/j.ijast.20240803.11

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  • @article{10.11648/j.ijast.20240803.11,
      author = {Sayid Ali and Tamrat Degefa and Asnaku Funga and Mosisa Dire and Ayda Mohamed and Asmarech Yeshaneh},
      title = {Reproductive Biotechnology Tools as a Game Changer for the Dairy Sector: The Case of Sexed Semen and Estrus Synchronization to Produce Seedstock Dairy Heifers
    },
      journal = {International Journal of Animal Science and Technology},
      volume = {8},
      number = {3},
      pages = {30-37},
      doi = {10.11648/j.ijast.20240803.11},
      url = {https://doi.org/10.11648/j.ijast.20240803.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20240803.11},
      abstract = {Human curiosity about the sexual orientation of living things has a long history. For thousands of years, livestock owners wanted to find a suitable way to predict the sex of the progeny to be born for their herds on their farm. Thanks to the development of semen sexing technology, animal breeding has undergone a revolution, which allows farmers to control the sex of their offspring. Sexed semen enhances overall productivity and also assists farmers in meeting the rising demand for high-quality dairy animals by enabling them to selectively generate seedstock female calves. The technology is a one-stop solution to enhance breed improvement especially in a country like Ethiopia where 97 percent of the cattle population is indigenous, which is not selected for milk production and results in undernourishment for animal source origin diet, including milk and meat. The benefits of using sexed semen include sex selection, improved genetic selection, enhanced breeding effectiveness, better control over herd dynamics, greater financial gains, environmental sustainability, and improved animal welfare. Hence, with the finding of this study, an overall result of a 98% response rate 95% female skewness, and 79% conception rate was recorded after being inseminated with sexed semen. The finding of the response rate is closer to the results reported by another researcher, while the average conception rate is comparatively higher. This could be due to careful animal selection, the use of fertile quality semen, proper heat detection, and inseminating at an optimum window of time. In general, from the piece of this study, it is possible to foresee and conclude that the adoption and application of sexed semen technology is a groundbreaking and game-changer technology for the dairy industry in a country like Ethiopia, which overcomes the limitations of improved crossbred dairy cattle, replacement heifers, and high milk prices on the one hand, a shortage of grazing land, and environmental destruction due to a decreasing number of unwanted cattle population on the other side.
    },
     year = {2024}
    }
    

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    AU  - Sayid Ali
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    AU  - Asmarech Yeshaneh
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    AB  - Human curiosity about the sexual orientation of living things has a long history. For thousands of years, livestock owners wanted to find a suitable way to predict the sex of the progeny to be born for their herds on their farm. Thanks to the development of semen sexing technology, animal breeding has undergone a revolution, which allows farmers to control the sex of their offspring. Sexed semen enhances overall productivity and also assists farmers in meeting the rising demand for high-quality dairy animals by enabling them to selectively generate seedstock female calves. The technology is a one-stop solution to enhance breed improvement especially in a country like Ethiopia where 97 percent of the cattle population is indigenous, which is not selected for milk production and results in undernourishment for animal source origin diet, including milk and meat. The benefits of using sexed semen include sex selection, improved genetic selection, enhanced breeding effectiveness, better control over herd dynamics, greater financial gains, environmental sustainability, and improved animal welfare. Hence, with the finding of this study, an overall result of a 98% response rate 95% female skewness, and 79% conception rate was recorded after being inseminated with sexed semen. The finding of the response rate is closer to the results reported by another researcher, while the average conception rate is comparatively higher. This could be due to careful animal selection, the use of fertile quality semen, proper heat detection, and inseminating at an optimum window of time. In general, from the piece of this study, it is possible to foresee and conclude that the adoption and application of sexed semen technology is a groundbreaking and game-changer technology for the dairy industry in a country like Ethiopia, which overcomes the limitations of improved crossbred dairy cattle, replacement heifers, and high milk prices on the one hand, a shortage of grazing land, and environmental destruction due to a decreasing number of unwanted cattle population on the other side.
    
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