Volume 3, Issue 1, March 2019, Page: 1-6
Isolation and Characterization of L- Asparaginase Producing Endophytic Bacteria from Simarouba Gluaca
Pathuppilly Satheesan Kavya, Center for Biotechnology Engineering, MET’S School of Engineering, Thrissur, India
Parakkottil Chothi Madhu, Center for Biotechnology Engineering, MET’S School of Engineering, Thrissur, India
Received: Nov. 10, 2018;       Accepted: Jan. 14, 2019;       Published: Feb. 7, 2019
DOI: 10.11648/j.ijast.20190301.11      View  15      Downloads  8
Abstract
Cancer cells differentiate themselves from normal cells in diminished expression of L-asparaginase. It is the enzyme that catalyzes the hydrolysis of L-Asparagine to L-aspartic and ammonia, because of these it is used as a medication and in food manufacturing. As a medication L-aspraginase is used to treat various types of leukemia such as, acute lymphoblastic leukemia, acute myeloma leukemia and non Hodgkin’s lymphoma. Hence they are not capable of producing L-asparaginase and mainly depend on the L-asparagine from circulating plasma pools. The clinical action of this enzyme is attributed to the reduction of L-asparaginase, since tumor cells unable to synthesis these amino acids are selectively killed by L-asparaginase depravation. This enzyme is widely distributed, being found in as it is widely distributed bacteria as well as found in animals, microbes and plant sources. In the present study L-asparaginase producing bacteria was isolated from Simarouba glauca. It was grown on the modified M9 medium in which L- asparagine was the major source of L- asparaginase production was detected by the formation of pink colored zones on the medium. After the partial purification of the L-Asparaginase enzyme, the enzyme activity was found to be 155.83 Units/ml and specific activity of 779.15. The optimum pH was to be found at pH 8 at a temperature of 37°C in the presence of 10mM Mg2+. The molecular identification was done by16S rDNA, PCR and sequence analysis by BLAST further confirmed that Bacillus cereus.
Keywords
L-Asparaginase, Simarouba Glauca, Bacillus Cereus, Enzyme Specific Activity, Cancer Cells
To cite this article
Pathuppilly Satheesan Kavya, Parakkottil Chothi Madhu, Isolation and Characterization of L- Asparaginase Producing Endophytic Bacteria from Simarouba Gluaca, International Journal of Animal Science and Technology. Vol. 3, No. 1, 2019, pp. 1-6. doi: 10.11648/j.ijast.20190301.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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