Volume 2, Issue 3, September 2018, Page: 30-35
Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers
Drissa Konaté, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali
Diakaridia Traoré, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali; National Center for Artificial Insemination, Bamako, Mali
Sognan Dao, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali
Rokiatou Fané, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali
Oumar Ouattara, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali
Ramata Diop, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali
Amadou Hamadoun Babana, Department of Biology, Faculty of Sciences & Techniques, University of Sciences, Techniques and Technologies, Bamako, Mali
Received: Sep. 22, 2018;       Accepted: Oct. 15, 2018;       Published: Nov. 13, 2018
DOI: 10.11648/j.ijast.20180203.11      View  183      Downloads  24
The N'Dama race, trypanotolerant and well adapted to the climatic conditions of Mali, is threatened of disappearance by the introduction of other genes by means of the artificial insemination with the exotic races or zebus. In order to adopt preservation and conservation strategies, it is important to study the genetic characteristics of the race across the country. In this study, carried out on the race in its cradle (Yanfolila district, Sikasso region), the genetic diversity of 119 N'Dama race, from the Madina-Diassa Center for Preservation, Multiplication and Dissemination of Endemic Ruminant Livestock, was evaluated with 9 microsatellite markers (SSR). A total of 60 alleles were obtained. The number of alleles varied from 2 (BM 1824) to 12 (INRA 37) with an average of 6.67 per locus. The PIC ranked from 0.39 (BM 1824) to 0.9183 (INRA 37) with an average of 0.6605. Genetic diversity ranged from 0.4293 (BM 1824) to 0.9228 (INRA) with an average of 0.6908. The 119 N'Dama races were classified into two groups I and J according to the genetic similarities revealed by the 9 SSR markers using the UPGMA method. Group J was formed with the majority (85%) of individuals and composed of four (4) clusters J1, J2, J3 and J4. The 69 Nd, 71 Nd and 72 Nd individuals showed strong dissimilar compared to other individuals in group J and formed cluster J1. Moreover, one cluster, with 15% of individuals, was belonged to Group I. The results of this study will contribute to the application of molecular tools and strengthen strategies for conservation, preservation and genetic improvement of the N’Dama race in Mali.
Genetic Diversity, N'Dama, SSR Marker, Mali
To cite this article
Drissa Konaté, Diakaridia Traoré, Sognan Dao, Rokiatou Fané, Oumar Ouattara, Ramata Diop, Amadou Hamadoun Babana, Genetic Diversity of the N’Dama Breed in Mali Using SSR Markers, International Journal of Animal Science and Technology. Vol. 2, No. 3, 2018, pp. 30-35. doi: 10.11648/j.ijast.20180203.11
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